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plc inhibitor u73122  (Tocris)


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    Tocris plc inhibitor u73122
    Plc Inhibitor U73122, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plc+inhibitor+u73122/U+73122/pm41903111-156-1-4
    Average 96 stars, based on 737 article reviews
    plc inhibitor u73122 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Planar Chromatography:

    Article Title: Hyperactivity with disrupted attention by activation of an astrocyte synaptogenic cue
    Article Snippet: The following inhibitors and antagonists were used: Tetrodotoxin (Cayman Chemical 14964), CdCl 2 (Sigma Aldrich 439800), Nimodipine (Tocris Bioscience CAS 0600), CGP55845 (Tocris Bioscience 1248), Bicuculline (Abcam ab120110 or Tocris Bioscience 0131) and NNC711 (Tocris Bioscience 1779). .. PLC inhibitor U73122 (Tocris Bioscience 1268) or its control analog U73433 (Tocris Bioscience 4133) was applied in bath for 40 min prior to recording. .. 1,2-Bis(2-Aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA from Sigma Aldrich A4926) or Recombinant Light Chain from Tetanus Toxin (List Biological Laboratories 650A) was dialyzed in MSN through patch pipette prior to MSN depolarization at least for 20 min or 10 min, respectively.

    Article Title: Regulation of Focal Adhesion Dynamics and Cell Migration by PLC/PI3K-Mediated Metabolism of PtdIns (4,5) P2 in a Breast Cancer Cell Line
    Article Snippet: .. Stocks of PLC inhibitor U73122 (TOCRIS Cat. No. 1268), U-73343 inactive analog of U73122 (TOCRIS Cat. No. 4133), PI3K inhibitors LY294002 (TOCRIS Cat. No. 1130), LY 303511 inactive analog of LY294002 (TOCRIS Cat. No. 2418), DAG inhibitor R59-022 (TOCRIS Cat. No. 2194) and PKC inhibitor Go6976, (TOCRIS Cat. No. 2253) were prepared by dissolving them in DMSO according to their product information sheet instruction. .. These inhibitors were aliquoted into labeled sterile eppendorf tubes in a culture hood, then stored at -20 °C.

    Article Title: Signal Transduction Mechanisms for Glucagon-Induced Somatolactin Secretion and Gene Expression in Nile Tilapia ( Oreochromis niloticus ) Pituitary Cells
    Article Snippet: .. Pharmacological agents, including the adenylyl cyclase inhibitor MDL12330A, a PKA-specific inhibitor H89, a selective activator of adenylyl cyclase, a cAMP analog 8-Bromo-cAMP, a non-selective phosphodiesterase inhibitor IBMX, a PLC activator m-3M3FBS, a PLC inhibitor U73122, a selective IP3 receptor antagonist, PKC inhibitors GF109203X and calphostin C, a VSCC blocker nifedipine, a SERCA inhibitor thapsigargin, a CaM antagonist calmidazolium, and a CaM-dependent protein kinase-II (CaMK-II) inhibitor KN62 were obtained from Tocris (Ellisville, Missouri, USA). ..

    Article Title: Caveolin-1 regulates medium spiny neuron structural and functional plasticity.
    Article Snippet: .. For experiments with AAV (University of Minnesota Viral Core), or PLC inhibitor U73122 (Tocris), 1 μl AAV, or 100 nM U73122 was added during the media change at DIV4. ..

    Article Title: Human B cells infected by Trypanosoma cruzi undergo F-actin disruption and cell death via caspase-7 activation and cleavage of phospholipase Cγ1.
    Article Snippet: B cells contribute to the immune system in many ways such as antigen presentation to CD4 T cells, secretion of cytokines and lymphoid tissue organogenesis.. Furthermore, they are the only cell type capable of producing immunoglobulins.. B cells also account for critical aspects of the resistance against intracellular pathogens.

    Article Title: Mechanisms of norepinephrine induced Ca 2+ responses in perivascular adipose tissue.
    Article Snippet: .. The PLC inhibitor U73122 (Tocris, 1268, 10μM) decreased Ca2+ responses in WAT, while it 301 increased Ca2+ responses in mPVAT and had no significant effect on aPVAT (Fig. 4). ..

    Control:

    Article Title: Hyperactivity with disrupted attention by activation of an astrocyte synaptogenic cue
    Article Snippet: The following inhibitors and antagonists were used: Tetrodotoxin (Cayman Chemical 14964), CdCl 2 (Sigma Aldrich 439800), Nimodipine (Tocris Bioscience CAS 0600), CGP55845 (Tocris Bioscience 1248), Bicuculline (Abcam ab120110 or Tocris Bioscience 0131) and NNC711 (Tocris Bioscience 1779). .. PLC inhibitor U73122 (Tocris Bioscience 1268) or its control analog U73433 (Tocris Bioscience 4133) was applied in bath for 40 min prior to recording. .. 1,2-Bis(2-Aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA from Sigma Aldrich A4926) or Recombinant Light Chain from Tetanus Toxin (List Biological Laboratories 650A) was dialyzed in MSN through patch pipette prior to MSN depolarization at least for 20 min or 10 min, respectively.

    Bioprocessing:

    Article Title: Caveolin-1 regulates medium spiny neuron structural and functional plasticity.
    Article Snippet: .. For experiments with AAV (University of Minnesota Viral Core), or PLC inhibitor U73122 (Tocris), 1 μl AAV, or 100 nM U73122 was added during the media change at DIV4. ..



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    PIP 2 attenuation upon loss of Pfn1 is reversible by PLC inhibition. (A,B) Representative PIP 2 staining images (A; 40×) of control and Pfn1 KO MDA 231 cells, treated with either inactive ( U73343 ) or active <t>(U73122)</t> pan-PLC inhibitor drug (magnified images of PIP 2 staining of selected regions of interest are shown alongside). (B) The associated PM PIP 2 quantification for the various groups (all data normalized to the average readout of control cells treated with the U73343 compound). A total of ∼450 cells/group were analyzed from three independent experiments. Scale bar: 40 μm. (C–E) Representative PLCβ3 and Pfn1 immunoblots of lysates of MDA-231 cells following transient transfection of the indicated siRNAs (non-targeting control siRNA transfection served as control); GAPDH blot serves as the loading control (C). Representative PIP 2 staining images fluorescence images (40×) of MDA-231 cells transfected with the indicated siRNAs (magnified images of PIP 2 staining of selected regions of interest of each group are shown underneath) (D). Scale bar: 80 μm. (E) Associated quantification to compare the PM PIP 2 intensity between the various groups of cells based on the results of three independent experiments. In B,E, the box represents the data falling within the 25th to 75th percentile range, and a line denoting the median value of the data set. Whiskers represent 1.5× the interquartile range above and below the 25th and 75th percentiles. * P <0.05; *** P <0.001 (one-way ANOVA with Tukey HSD post-hoc test for multiple comparisons).
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    PIP 2 attenuation upon loss of Pfn1 is reversible by PLC inhibition. (A,B) Representative PIP 2 staining images (A; 40×) of control and Pfn1 KO MDA 231 cells, treated with either inactive ( U73343 ) or active <t>(U73122)</t> pan-PLC inhibitor drug (magnified images of PIP 2 staining of selected regions of interest are shown alongside). (B) The associated PM PIP 2 quantification for the various groups (all data normalized to the average readout of control cells treated with the U73343 compound). A total of ∼450 cells/group were analyzed from three independent experiments. Scale bar: 40 μm. (C–E) Representative PLCβ3 and Pfn1 immunoblots of lysates of MDA-231 cells following transient transfection of the indicated siRNAs (non-targeting control siRNA transfection served as control); GAPDH blot serves as the loading control (C). Representative PIP 2 staining images fluorescence images (40×) of MDA-231 cells transfected with the indicated siRNAs (magnified images of PIP 2 staining of selected regions of interest of each group are shown underneath) (D). Scale bar: 80 μm. (E) Associated quantification to compare the PM PIP 2 intensity between the various groups of cells based on the results of three independent experiments. In B,E, the box represents the data falling within the 25th to 75th percentile range, and a line denoting the median value of the data set. Whiskers represent 1.5× the interquartile range above and below the 25th and 75th percentiles. * P <0.05; *** P <0.001 (one-way ANOVA with Tukey HSD post-hoc test for multiple comparisons).
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    PIP 2 attenuation upon loss of Pfn1 is reversible by PLC inhibition. (A,B) Representative PIP 2 staining images (A; 40×) of control and Pfn1 KO MDA 231 cells, treated with either inactive ( U73343 ) or active <t>(U73122)</t> pan-PLC inhibitor drug (magnified images of PIP 2 staining of selected regions of interest are shown alongside). (B) The associated PM PIP 2 quantification for the various groups (all data normalized to the average readout of control cells treated with the U73343 compound). A total of ∼450 cells/group were analyzed from three independent experiments. Scale bar: 40 μm. (C–E) Representative PLCβ3 and Pfn1 immunoblots of lysates of MDA-231 cells following transient transfection of the indicated siRNAs (non-targeting control siRNA transfection served as control); GAPDH blot serves as the loading control (C). Representative PIP 2 staining images fluorescence images (40×) of MDA-231 cells transfected with the indicated siRNAs (magnified images of PIP 2 staining of selected regions of interest of each group are shown underneath) (D). Scale bar: 80 μm. (E) Associated quantification to compare the PM PIP 2 intensity between the various groups of cells based on the results of three independent experiments. In B,E, the box represents the data falling within the 25th to 75th percentile range, and a line denoting the median value of the data set. Whiskers represent 1.5× the interquartile range above and below the 25th and 75th percentiles. * P <0.05; *** P <0.001 (one-way ANOVA with Tukey HSD post-hoc test for multiple comparisons).
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    PIP 2 attenuation upon loss of Pfn1 is reversible by PLC inhibition. (A,B) Representative PIP 2 staining images (A; 40×) of control and Pfn1 KO MDA 231 cells, treated with either inactive ( U73343 ) or active <t>(U73122)</t> pan-PLC inhibitor drug (magnified images of PIP 2 staining of selected regions of interest are shown alongside). (B) The associated PM PIP 2 quantification for the various groups (all data normalized to the average readout of control cells treated with the U73343 compound). A total of ∼450 cells/group were analyzed from three independent experiments. Scale bar: 40 μm. (C–E) Representative PLCβ3 and Pfn1 immunoblots of lysates of MDA-231 cells following transient transfection of the indicated siRNAs (non-targeting control siRNA transfection served as control); GAPDH blot serves as the loading control (C). Representative PIP 2 staining images fluorescence images (40×) of MDA-231 cells transfected with the indicated siRNAs (magnified images of PIP 2 staining of selected regions of interest of each group are shown underneath) (D). Scale bar: 80 μm. (E) Associated quantification to compare the PM PIP 2 intensity between the various groups of cells based on the results of three independent experiments. In B,E, the box represents the data falling within the 25th to 75th percentile range, and a line denoting the median value of the data set. Whiskers represent 1.5× the interquartile range above and below the 25th and 75th percentiles. * P <0.05; *** P <0.001 (one-way ANOVA with Tukey HSD post-hoc test for multiple comparisons).
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    Image Search Results


    PIP 2 attenuation upon loss of Pfn1 is reversible by PLC inhibition. (A,B) Representative PIP 2 staining images (A; 40×) of control and Pfn1 KO MDA 231 cells, treated with either inactive ( U73343 ) or active (U73122) pan-PLC inhibitor drug (magnified images of PIP 2 staining of selected regions of interest are shown alongside). (B) The associated PM PIP 2 quantification for the various groups (all data normalized to the average readout of control cells treated with the U73343 compound). A total of ∼450 cells/group were analyzed from three independent experiments. Scale bar: 40 μm. (C–E) Representative PLCβ3 and Pfn1 immunoblots of lysates of MDA-231 cells following transient transfection of the indicated siRNAs (non-targeting control siRNA transfection served as control); GAPDH blot serves as the loading control (C). Representative PIP 2 staining images fluorescence images (40×) of MDA-231 cells transfected with the indicated siRNAs (magnified images of PIP 2 staining of selected regions of interest of each group are shown underneath) (D). Scale bar: 80 μm. (E) Associated quantification to compare the PM PIP 2 intensity between the various groups of cells based on the results of three independent experiments. In B,E, the box represents the data falling within the 25th to 75th percentile range, and a line denoting the median value of the data set. Whiskers represent 1.5× the interquartile range above and below the 25th and 75th percentiles. * P <0.05; *** P <0.001 (one-way ANOVA with Tukey HSD post-hoc test for multiple comparisons).

    Journal: Journal of Cell Science

    Article Title: Molecular insights into profilin 1-dependent regulation of cellular phosphatidylinositol (4,5)-bisphosphate

    doi: 10.1242/jcs.265025

    Figure Lengend Snippet: PIP 2 attenuation upon loss of Pfn1 is reversible by PLC inhibition. (A,B) Representative PIP 2 staining images (A; 40×) of control and Pfn1 KO MDA 231 cells, treated with either inactive ( U73343 ) or active (U73122) pan-PLC inhibitor drug (magnified images of PIP 2 staining of selected regions of interest are shown alongside). (B) The associated PM PIP 2 quantification for the various groups (all data normalized to the average readout of control cells treated with the U73343 compound). A total of ∼450 cells/group were analyzed from three independent experiments. Scale bar: 40 μm. (C–E) Representative PLCβ3 and Pfn1 immunoblots of lysates of MDA-231 cells following transient transfection of the indicated siRNAs (non-targeting control siRNA transfection served as control); GAPDH blot serves as the loading control (C). Representative PIP 2 staining images fluorescence images (40×) of MDA-231 cells transfected with the indicated siRNAs (magnified images of PIP 2 staining of selected regions of interest of each group are shown underneath) (D). Scale bar: 80 μm. (E) Associated quantification to compare the PM PIP 2 intensity between the various groups of cells based on the results of three independent experiments. In B,E, the box represents the data falling within the 25th to 75th percentile range, and a line denoting the median value of the data set. Whiskers represent 1.5× the interquartile range above and below the 25th and 75th percentiles. * P <0.05; *** P <0.001 (one-way ANOVA with Tukey HSD post-hoc test for multiple comparisons).

    Article Snippet: In experiments involving pan-PLC inhibitor U73122 and its inactive control U73343 (Medchem Express, # HY108630 ), the dry compounds were diluted in DMSO to a stock concentration of 10 mM and added to cells at a final concentration of 2 μM after 24 h of serum stimulation.

    Techniques: Inhibition, Staining, Control, Western Blot, Transfection, Fluorescence